Surgical Research Laboratory
P. Jack Hoopes, DVM, PhD
Professor of Surgery and Medicine, The Geisel School of Medicine at Dartmouth
Adjunct Professor of Biomedical Engineering, Thayer School of Engineering, Dartmouth College
The Surgical Research Laboratory (SRL) is a 10,000 sq. ft. bench research laboratory and experimental animal OR research facility operated under the direction of the Department of Surgery (DOS). The SRL is directed by DOS staff member P. Jack Hoopes, DVM, PhD. The SRL receives administrative input and oversight from the Geisel School of Medicine, Dartmouth College's Animal Care and Use Program, and the Institutional Animal Care and Use Committee (IACUC). The full-time SRL support staff includes two veterinarians, two veterinary technicians, two research associates, three laboratory/OR managers, and a part-time financial manager. An SRL resident faculty and staff of more than 25 currently support the research of 15 graduate students and post doctoral fellows and more than 30 undergraduates annually.
The basic science component of the SRL includes a complete array of molecular biology instrumentation and techniques including: cell culture, DNA microarray, proteomics array, northern, western, and southern blots, ELISA, TUNNEL assay, RT-PCR, autoradiography, etc. The SRL has dedicated expertise in histologic preparation and staining/labeling techniques including histochemistry, immunohistochemistry, in situ-hybridization as well as fluorescent microscopy, and automated/computer-based microscopic image analysis/quantification.
The experimental animal operating suite routinely studies species ranging from rodents and hamsters to all commonly used large research models including spontaneous canine tumors (pet dogs) treated with curative intent on an NIH/NCI grant protocol in preparation for an upcoming nanotechnology-based breast cancer clinical being directed by NCCC Deputy Director and DOS attending Burton Eisenberg, MD and Professor Jack Hoopes.
- SRL surgical and imaging techniques include state-of-the-art animal anesthesia delivery and monitoring, dedicated clinical fluoroscopy/angiography, ultrasound and a laser, and ionizing radiation laboratory.
- The facility contains five permanent and two mobile operating microscopes, suitable for conventional and microsurgery applications.
- NCCC Director, Mark Israel, MD, SRL Director, Professor Jack Hoopes, and research colleagues recently acquired an NIH-NCI shared resource grant to construct and further develop a small animal imaging resource at NCCC/DHMC. This shared resource, which is currently under construction, will house rodent dedicated MRI, CT PET, ultrasound, fluoroscopy/angiography and bioluminescence, and fluorescence imaging instrumentation.
- The same instrumentation is currently available for large animal imaging. Expertise and instrumentation for endoscopy, laparoscopy and radiation therapy/treatment planning are also available. Taken together, research animal-based imaging and surgery technology and instrumentation is at the forefront of the national research effort in this area.
- The NIH-DHMC supported Advanced Surgical Center (ASC) which was funded in 2010 is now under construction (slated for completion in March, 2013). This facility, one of fifteen in the USA, is a two room OR facility for clinical patients and selected research animal subjects, which included built-in intraoperative MRI, CT, and bi-planar fluroscopic/angiography technology. The Dartmouth facility is the only one in the USA and Canada to be dual-fitted for animal-based surgical research and clinical patients.